Cat.No.
ULP1-1571S
Species
Saccharomyces cerevisiae
Product Name
Recombinant S. cerevisiae ULP1 protein
Product Overview
Recombinant protein Saccharomyces cerevisiae (yeast) Ulp1 was expressed in E. coli.
Description
Yeast Ulp1 is a highly active protease which specifically recognizes the tertiary structure of ubiquitin-like protein, SUMO rather than a short sequence as other
commonly used proteases such as Thrombin, TEV, and EK. The protease can be used to cleave the SUMO from recombinant fusion proteins at 30 centigrade or 4 centigrade at pH 7.0-7.9. After digestion, the His-Tagged Ulp1 can be easily removed by His tag affinity chromatography.
commonly used proteases such as Thrombin, TEV, and EK. The protease can be used to cleave the SUMO from recombinant fusion proteins at 30 centigrade or 4 centigrade at pH 7.0-7.9. After digestion, the His-Tagged Ulp1 can be easily removed by His tag affinity chromatography.
Source
E. coli
Form
50 mM Tris-HCl, (pH7.5), 150 mM NaCl, 1 mM DTT, and 50% glycerol.
Molecular Mass
27 kDa
Storage
Stable for 2 years at -70 centigrade from date of shipment. Please aliquot to avoid repeated freezing and thawing.
Official Symbol
Synonyms
ULP1; SUMO protease ULP1
UniProt ID
Unit Definition
1U cleaves 85% of 100 ug of sumo-fusion protein at 30 centigrade for 1 hour or 80% at 4 centigrade overnight in a buffer containing 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM DTT, and 10% glycerol.
Gene ID
mRNA Refseq
Protein Refseq
