Cat.No.
mutM-52E
Species
E. coli
Product Name
Recombinant E.coli mutM
Product Overview
A recombinant E. coli strain carrying the cloned fpg gene.
Description
Fpg (also known as Formamidopyrimidine DNA glycosylase, Mut M, FAPY DNA Glycosylase, and 8-oxoguanine DNA glycosylase) participates in the base-excision (BER) pathway of DNA repair enzymes and acts both as a N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged purines from double stranded DNA, generating an apurinic/apyrimidinic (AP site). The AP-lyase activity cleaves both the 3′ and 5′ phosphodiester bonds at the AP site, producing a 1 base gap in the DNA and 3′ and 5′ phosphate termini. Bases recognized and removed by Fpg include 7, 8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, fapy-guanine, methy-fapy-guanine, fapy-adenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine and 5-hydroxy-uracil.
Source
E. coli
Bio-activity
20,513 U/mg
Molecular Mass
30,290 Daltons
Purity
>99% by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to cleave 1 pmol of a 34mer oligo-nucleotide duplex containing an 8-oxoguanine base paired with a cysteine in 1 hour at 37°C in a total reaction volume of 10μl in reaction buffer.
Storage
-25 to -15°C
Concentration
8,000 U/ml
Storage Buffer
Supplied in: 20mM Tris-HCL, 50 mM KCl, 1mM DTT, 0.1mM EDTA, 50% glycerol pH 8.0 @ 25°C. Supplied with: 10X Yellow Buffer: 100mM Bis-Tris Propane, 100mM MgCl2, 10mM DTT pH 7.0 @ 25°C.
Official Symbol
mutM
Synonyms
mutM; formamidopyrimidine/5-formyluracil/ 5-hydroxymethyluracil DNA glycosylase; ECK3625; fpg; JW3610; NP_418092.1; EC 3.2.2.23; formamidopyrimidine DNA glycosylase
Gene ID
Protein Refseq
