Cat.No.
Mic-0051M
Product Name
Phenol Red Sucrose Broth
Product Details
Phenol Red Sucrose Broth is a liquid medium recommended to differentiate microorganisms based on their ability to ferment sucrose. Proteose Peptone and Beef Extract provide nitrogenous compounds, carbon, vitamins and trace elements to the microorganisms, while Sodium Chloride serves as source of essential electrolytes and maintains the osmotic equilibrium. All members of the Enterobacteriaceae family can use Sucrose as an energy source, and therefore grow well in this medium. Sucrose fermentation produces gases and organic acids, being butyric acid the main constituent. The pH indicator Phenol Red shifts from red to yellow upon accumulation of these products, thus revealing Sucrose fermentation. Additionally, production of butyric acid can be recognized by a pungent, foul odor.
Product Ingredients
Casein Digest Peptone (10.0 g/L), Sodium Chloride (5.0 g/L), Phenol Red (0.018 g/L), Sucrose 5.0 g/L). Final pH: 7.4 ± 0.2 at 25 °C. Grams per liter may be adjusted or formula supplemented to obtain desired performance.
size
500g, 2kg, 10kg
Usage Method
Mix 20 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 116-118°C for 15 minutes.
Application
Food safety
Storage
Store the sealed bottle containing the dehydrated medium at 2 to 30°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original color.
Data Sheet
