MacConkey Agar

Mic-0037M

Product Name

MacConkey Agar

Product Details

MacConkey Agar is a solid medium recommended for the cultivation of Gram-negative, lactose-fermenting enteric bacteria in samples of water, milk, and foods. MacConkey Agar is used for the isolation and differentiation of the coliform organisms and enteric pathogens based upon the fermentation of Lactose. This formulation is an improvement on the original. Modifications were made to improve the growth of Salmonella and Shigella strains. MacConkey Agar contains Crystal Violet and Bile Salts that inhibit Gram-positive organisms and allow Gram-negative organisms to grow. Due to the presence of the Neutral Red pH indicator, coliforms that ferment Lactose turn dark red to purple color while non-fermenters are colorless.

Product Ingredients

Pancreatic Digest of Gelatin (17.0g/L), Enzymatic Digest of Casein (1.5g/L), Meat Peptone (1.5g/L), Neutral Red (0.03g/L), Lactose (10.0g/L), Sodium Chloride (5.0g/L), Agar (13.5g/L), Bile Salts #3 (1.5g/L), Crystal Violet (0.001g/L). Final pH: 7.1 ± 0.2 at 25°C. Grams per liter may be adjusted or formula supplemented to obtain desired performance.

size

500g, 2kg, 10kg

Usage Method

Mix 50 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121°C for 15 minutes.

Application

Food safety
Cosmetic industry

Storage

Store the sealed bottle containing the dehydrated medium at 2 to 30°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light pink to beige color.