Cat.No.
Mic-0032M
Product Name
Listeria Enrichment Broth
Product Details
Listeria Enrichment Broth is used for the selective enrichment and isolation of Listeria spp. This medium was developed for the recovery of Listeria monocytogenes from cheeses and pasteurized milk. Peptones and Yeast Extract supply necessary growth nutrients such as amino acids, peptides, carbohydrates, and vitamins. Dextrose is an energy source for the bacteria. Sodium Chloride provides essential electrolytes and maintains osmotic equilibrium. Potassium Phosphate, Dibasic acts as a buffering agent. Selectivity for Listeria is accomplished by the addition of Cycloheximide, Nalidixic acid and Acriflavine. Subculturing of enrichment broth onto Listeria selective agar plates is recommended to increase isolation rates.
Product Ingredients
Enzymatic Digest of Casein (17.0g/L), Yeast Extract (6.0g/L), Soy Peptone (3.0g/L), Dextrose (2.5g/L), Sodium Chloride (5.0g/L), Potassium Phosphate, Dibasic (2.5g/L), Cycloheximide (0.05g/L), Nalidixic Acid (0.04g/L), Acriflavine, HCI (0.015g/L). Final pH: 7.3 ± 0.2 at 25°C. Grams per liter may be adjusted or formula supplemented to obtain desired performance.
size
500g, 2kg, 10kg
Usage Method
Mix 36.1 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring to dissolve completely. Autoclave at 121°C for 15 minutes.
Application
Food safety
Storage
Store the sealed bottle containing the dehydrated medium at 2 to 30°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original color.
Data Sheet
