Cat.No.
Mic-0033M
Product Name
Lee’s Multi-Differential Agar (LMDA)
Product Details
Lee’s Multi-Differential Agar (LMDA) is a differential solid medium for the detection and enumeration of a variety of brewery bacteria and yeast.
Product Ingredients
Dextrose (10.0 g/L), Bromocresol Green Free Acid (0.022 g/L), Citric Acid (1.1 g/L), Calcium Pantothenate (2.0 g/L), Monopotassium Phosphate (0.5 g/L), Manganese Sulfate (0.01 g/L), Sodium Chloride (0.01 g/L), Ferrous Sulfate (0.01 g/L), Tomato Juice Broth (20.0 g/L), Tween 80 (0.5 g/L), Yeast Extract (10.0 g/L), Calcium Carbonate (5.0 g/L), Peptonized Milk (20.0 g/L), Dipotassium Phosphate (0.5 g/L), Magnesium Sulfate (0.2 g/L), Agar (15.0 g/L). Final pH: 5.5 ± 0.2 at 25 °C. Grams per liter may be adjusted or formula supplemented to obtain desired performance.
size
500g, 2kg, 10kg
Usage Method
Mix 84.8 grams of the medium in one liter of purified water until evenly dispersed. Heat with repeated stirring and boil to dissolve completely. Additionally, 0.007g of cycloheximide can be added to prevent the growth of yeast. Autoclave at 121°C for 15 minutes. Cool medium to approximately 45-50°C in a water bath. Gently and thoroughly swirl the medium, without agitation, in order to uniformly suspend the calcium carbonate precipitate. Dispense the medium into sterile petri dishes. Immediate use after preparation is recommended to prevent pH changes.
Application
Brewing industry
Microbiology
Microbiology
Storage
Store the sealed bottle containing the dehydrated medium at 2 to 30°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original color.
Data Sheet
